Efek ekstrak etanol biji alpukat (persea americana) terhadap biofilm candida albicans

Background: Candida albicans is the most common bacteria related to root canal treatment failure. Avocado seeds (Persea americana) contain terpenoids, alkaloids, flavonoids, steroids, saponins and tannins that have antibacterial activity. Purpose: To determine effect of avocado seed ethanol extract on C.albicans biofilm. Methods: The ATCC 10231 biofilm was evaluated by crystal violet assay. were inoculated into Sabouraud Dextrose Broth at 37C for 24 h. A volume 200 L (108 CFU/mL) culture distributed 96-well plates incubated diluted 6 concentrations: 100%, 50%, 25%, 12.5%, 6.25% 3.13% with NaOH 0.1N. NaOCl 5.25% used as positive control. added 1 h, 3 h biomass calculated 0,5% staining optical density value measured using a microplate reader (490 nm). Results: Ethanol all concentrations from each incubation period had an antibiofilm against when compared negative control (p < 0.05). concentration has highest which showed 0.011 ± 0.024. Conclusion: C.albicans.